TargetNet
P30291
Wee1-like protein kinase
Function
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Database Links
| Database | Links |
|---|---|
| UniProt | P30291 |
| PDB | 1X8B, 2IN6, 2IO6, 2Z2W, 3BI6, 3BIZ, 3CQE, 3CR0 |
| BioGrid | 113303 |
| BindingDB | P30291 |
| DrugBank | Not available |
| Guide to PHARMACOLOGY | 2278 |
| PharmGKB | PA366 |
| KEGG | hsa:7465 |
| BioCyc | Not available |
| Entrez Gene (GeneID) | 7465 |
Chemical Space of the Positive Compounds
Model Performance Metrics
| Metric | Mean Value | Standard Deviation |
|---|---|---|
| AUC | 0.9698 | 0.0025 |
| Accuracy | 0.9237 | 0.0041 |
| Sensitivity | 0.9197 | 0.0061 |
| Specificity | 0.9271 | 0.0055 |
| BEDROC | 0.9975 | 0.0025 |
| MCC Threshold | 0.542 | N/A |
| MCC | 0.8625 | N/A |
| F-score Threshold | 0.326 | N/A |
| F-score | 0.9256 | N/A |