DNA topoisomerase 1
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.
Database | Links |
---|---|
UniProt | P11387 |
PDB | 1A31, 1A35, 1A36, 1EJ9, 1K4S, 1K4T, 1LPQ, 1NH3, 1R49, 1RR8, 1RRJ, 1SC7, 1SEU, 1T8I, 1TL8 |
BioGrid | 113003 |
BindingDB | P11387 |
DrugBank | DB00762, DB04967, DB01030 |
Guide to PHARMACOLOGY | Not available |
PharmGKB | PA353 |
KEGG | hsa:7150 |
BioCyc | Not available |
Entrez Gene (GeneID) | 7150 |
Metric | Mean Value | Standard Deviation |
---|---|---|
AUC | 0.9417 | 0.0043 |
Accuracy | 0.8639 | 0.0073 |
Sensitivity | 0.8699 | 0.0082 |
Specificity | 0.8573 | 0.0127 |
BEDROC | 0.9828 | 0.0146 |
MCC Threshold | 0.706 | N/A |
MCC | 0.7494 | N/A |
F-score Threshold | 0.302 | N/A |
F-score | 0.8798 | N/A |